EIF3J Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97521

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EIF3J Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human EIF3J fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for EIF3J Antibody

Western Blot: EIF3J Antibody [NBP2-97521]

Western Blot: EIF3J Antibody [NBP2-97521]

Western Blot: EIF3J Antibody [NBP2-97521] - Anti-EIF3J rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: U-251MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: EIF3J Antibody [NBP2-97521]

Immunocytochemistry/ Immunofluorescence: EIF3J Antibody [NBP2-97521]

Immunocytochemistry/Immunofluorescence: EIF3J Antibody [NBP2-97521] - Immunofluorescence staining of EIF3J in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human EIF3J polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: EIF3J Antibody [NBP2-97521]

Immunohistochemistry-Paraffin: EIF3J Antibody [NBP2-97521]

Immunohistochemistry-Paraffin: EIF3J Antibody [NBP2-97521] - Immunochemical staining of human EIF3J in human pancreas with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
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Applications for EIF3J Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:400

Immunoprecipitation

0.5-2uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EIF3J

Eukaryotic initiation factor-3 (EIF3) has a molecular mass of about 600 kD and contains 13 nonidentical protein subunits, including EIF3J. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex (Fraser et al., 2004 [PubMed 14688252]; Fraser et al., 2007 [PubMed 17588516]).[supplied by OMIM]

Alternate Names

eIF3 p35, eIF3-alpha, eIF3j, eIF3-p35, EIF3S1eIF-3-alpha, Eukaryotic translation initiation factor 3 subunit 1, eukaryotic translation initiation factor 3 subunit J, eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD), eukaryotic translation initiation factor 3, subunit 1 alpha, 35kDa, eukaryotic translation initiation factor 3, subunit J

Gene Symbol

EIF3J

Additional EIF3J Products

Product Documents for EIF3J Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EIF3J Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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