eIF4A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16302

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-16302
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Chimpanzee (100%), Mouse (100%), Porcine (100%), Rat (100%), Xenopus (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eIF4A1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF4A1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF4A1 Antibody

Western Blot: eIF4A1 Antibody [NBP2-16302]

Western Blot: eIF4A1 Antibody [NBP2-16302]

Western Blot: eIF4A1 Antibody [NBP2-16302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF4A1 antibody diluted by 1:3000.
Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody [NBP2-16302]

Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody [NBP2-16302]

Immunocytochemistry/Immunofluorescence: eIF4A1 Antibody [NBP2-16302] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-16302]

Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-16302]

Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-16302] - Human colon carcinoma, using EIF4A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
View 1 more images

Applications for eIF4A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF4A1

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Alternate Names

ATP-dependent RNA helicase eIF4A-1, DDX2Aeukaryotic initiation factor 4A-I, EC 3.6.1, EC 3.6.4.13, EIF4A, EIF-4A, eIF-4A-I, eIF4A-I, eukaryotic initiation factor 4AI, eukaryotic translation initiation factor 4A, eukaryotic translation initiation factor 4A, isoform 1, eukaryotic translation initiation factor 4A1

Gene Symbol

EIF4A1

UniProt

P60842 (Human)

Additional eIF4A1 Products

Product Documents for eIF4A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for eIF4A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.