eIF4A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24632

Novus Biologicals, part of Bio-Techne
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NBP2-24632
NBP2-24632SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Chicken, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all eIF4A1 Antibodies »

Product Specifications

Immunogen

A portion of amino acid 350-400 of human eIF4A1 was used as the immunogen.

Reactivity Notes

Zebrafish (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF4A1 Antibody

Western Blot: eIF4A1 Antibody [NBP2-24632]

Western Blot: eIF4A1 Antibody [NBP2-24632]

Western Blot: eIF4A1 Antibody [NBP2-24632] - Representative immunoblot of total lysate of MEFs with eIF4A (C-terminal epitope) and Tubulin antibodies in control and GS (glucose starvation). Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/21907) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-24632]

Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-24632]

Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-24632] - Analysis of eIF4A1 in human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
Western Blot: eIF4A1 Antibody [NBP2-24632]

Western Blot: eIF4A1 Antibody [NBP2-24632]

Western Blot: eIF4A1 Antibody [NBP2-24632] - Analysis of eIF4A1 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) RAW cell lysate in the absence of immunizing peptide using NBP2-24632 at 0.25 ug/ml.

Applications for eIF4A1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.25-1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF4A1

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Alternate Names

ATP-dependent RNA helicase eIF4A-1, DDX2Aeukaryotic initiation factor 4A-I, EC 3.6.1, EC 3.6.4.13, EIF4A, EIF-4A, eIF-4A-I, eIF4A-I, eukaryotic initiation factor 4AI, eukaryotic translation initiation factor 4A, eukaryotic translation initiation factor 4A, isoform 1, eukaryotic translation initiation factor 4A1

Gene Symbol

EIF4A1

UniProt

P60842 (Human)

Additional eIF4A1 Products

Product Documents for eIF4A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF4A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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