eIF4A2 Antibody (PSH01-89)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32301
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-89
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human eIF4A2 aa 1-407 / 407. (Uniprot: Q14240)
Localization
Cytosol.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for eIF4A2 Antibody (PSH01-89)
Western Blot: eIF4A2 Antibody (PSH01-89) [NBP3-32301]
Western Blot: eIF4A2 Antibody (PSH01-89) [NBP3-32301] - Western blot analysis of eIF4A2 on different lysates with Rabbit anti-eIF4A2 antibody (NBP3-32301) at 1/1,000 dilution.Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: PC-3M cell lysate
Lane 4: 293T cell lysate
Lane 5: MCF7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: C2C12 cell lysate
Lane 8: PC-12 cell lysate
Lane 9: COS-1 cell lysate
Lane 10: Human brain tissue lysate
Lane 11: Mouse testis tissue lysate
Lane 12: Rat testis tissue lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32301) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for eIF4A2 Antibody (PSH01-89)
Application
Recommended Usage
Immunoprecipitation
1:1000
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: eIF4A2
Alternate Names
ATP-dependent RNA helicase eIF4A-2, BM-010, DDX2Beukaryotic translation initiation factor 4A, isoform 2, EC 3.6.1, EC 3.6.4.13, EIF4A, eIF-4A-II, eIF4A-II, EIF4F, eukaryotic initiation factor 4A-II, eukaryotic translation initiation factor 4A, eukaryotic translation initiation factor 4A2
Gene Symbol
EIF4A2
Additional eIF4A2 Products
Product Documents for eIF4A2 Antibody (PSH01-89)
Product Specific Notices for eIF4A2 Antibody (PSH01-89)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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