EIF4A3 Antibody (6Y3N4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16727
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6Y3N4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 34-125 of human EIF4A3 (P38919). VDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EIF4A3 Antibody (6Y3N4)
Western Blot: EIF4A3 Antibody (6Y3N4) [NBP3-16727]
Western Blot: EIF4A3 Antibody (6Y3N4) [NBP3-16727] - Western blot analysis of extracts of various cell lines, using EIF4A3 Rabbit mAb (NBP3-16727) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunocytochemistry/ Immunofluorescence: EIF4A3 Antibody (6Y3N4) [NBP3-16727]
Immunocytochemistry/Immunofluorescence: EIF4A3 Antibody (6Y3N4) [NBP3-16727] - Immunofluorescence analysis of U-2 OS cells using EIF4A3 Rabbit mAb (NBP3-16727) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for EIF4A3 Antibody (6Y3N4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: EIF4A3
Alternate Names
ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DDX48, DEAD (Asp-Glu-Ala-Asp) box polypeptide 48, DEAD box protein 48, DKFZp686O16189, EC 3.6.1, EC 3.6.4.13, eIF4AIII, eIF-4A-III, eIF4A-III, eukaryotic initiation factor 4A-III, Eukaryotic initiation factor 4A-like NUK-34, eukaryotic translation initiation factor 4A, Eukaryotic translation initiation factor 4A isoform 3, eukaryotic translation initiation factor 4A3, hNMP 265, KIAA0111eukaryotic translation initiation factor 4A, isoform 3, MGC10862, NMP 265, NMP265, Nuclear matrix protein 265, NUK34
Gene Symbol
EIF4A3
Additional EIF4A3 Products
Product Documents for EIF4A3 Antibody (6Y3N4)
Product Specific Notices for EIF4A3 Antibody (6Y3N4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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