eIF4B [p Ser406] Antibody (JE58-32)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32762
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE58-32
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to residues surrounding Ser406 of human eIF4B protein. (Uniprot: P23588)
Modification
p Ser 406
Localization
cytosol, eukaryotic translation initiation factor 4F complex.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for eIF4B [p Ser406] Antibody (JE58-32)
![eIF4B [p Ser406] Antibody (JE58-32)](https://resources.bio-techne.com/images/products/nbp3-32762_rabbit-eif4b-p-ser406-mab-je58-32-288202412442972.jpg "Western Blot: eIF4B [p Ser406] Antibody (JE58-32) [NBP3-32762]")
Western Blot: eIF4B [p Ser406] Antibody (JE58-32) [NBP3-32762]
Western Blot: eIF4B [p Ser406] Antibody (JE58-32) [NBP3-32762] - Western blot analysis of eIF4B on different lysates with Rabbit anti-eIF4B antibody (NBP3-32762) at 1/2,000 dilution.Lane 1: NIH/3T3 cell lysate
Lane 2: HeLa cell lysate
Lane 3: NIH/3T3 treated pp for 1 hour cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 69 kDa
Observed band size: 80 kDa
Exposure time: 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32762) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for eIF4B [p Ser406] Antibody (JE58-32)
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: eIF4B
Long Name
Eukaryotic Translation Initiation Factor 4B
Alternate Names
Eif4a2
Gene Symbol
EIF4B
Additional eIF4B Products
Product Documents for eIF4B [p Ser406] Antibody (JE58-32)
Product Specific Notices for eIF4B [p Ser406] Antibody (JE58-32)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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