eIF4E Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-58833

Novus Biologicals, part of Bio-Techne
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NB100-58833
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Rabbit (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all eIF4E Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 167 and 217 of human eukaryotic translation initiation factor using the numbering given in entry NP_001959.1 (GeneID 1977).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF4E Antibody

Western Blot: eIF4E Antibody [NB100-58833]

Western Blot: eIF4E Antibody [NB100-58833]

Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: eIF4E Antibody [NB100-58833]

Immunoprecipitation: eIF4E Antibody [NB100-58833]

Immunoprecipitation: eIF4E Antibody [NB100-58833] - Detection of human eIF4E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4E antibody NB100-58833 used for IP at 3 ug per reaction. eIF4E was also immunoprecipitated by another rabbit anti-eIF4E antibody. For blotting immunoprecipitated eIF4E, NB100-58833 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for eIF4E Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 5 using NB100-58833 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: eIF4E

eIF4E is a protein that modulates the translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. . Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Long Name

Eukaryotic Translation Initiation Factor 4E

Alternate Names

EIF4EL1, EIF4F

Entrez Gene IDs

1977 (Human); 13684 (Mouse)

Gene Symbol

EIF4E

UniProt

P06730

Additional eIF4E Products

Product Documents for eIF4E Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for eIF4E Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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