eIF4E [p Ser209] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-81898
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Porcine
Cited:
Porcine
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).
Reactivity Notes
Porcine reactivity reported in scientific literature (PMID: 28183860).
Modification
p Ser209
Specificity
Detects endogenous levels of elF4E only when phosphorylated at serine 209.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for eIF4E [p Ser209] Antibody
Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody [NB100-81898] -
Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody [NB100-81898] - Staining of methanol-fixed MCF cells using NB100-81898
Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody [NB100-81898] -
Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody [NB100-81898] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81898 (left) or the same antibody preincubated with blocking peptide (right).Applications for eIF4E [p Ser209] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Immunohistochemistry
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:100
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: eIF4E
Long Name
Eukaryotic Translation Initiation Factor 4E
Alternate Names
EIF4EL1, EIF4F
Gene Symbol
EIF4E
UniProt
Additional eIF4E Products
Product Documents for eIF4E [p Ser209] Antibody
Product Specific Notices for eIF4E [p Ser209] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...