EIF4E2 Antibody (4G10)

Novus Biologicals, part of Bio-Techne | Catalog # H00009470-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4G10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EIF4E2 Antibodies »

Product Specifications

Immunogen

EIF4E2 (AAH05392, 1 a.a. ~ 245 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNNKFDALKDDDSGDHDQNEENSTQKDGEKEKTERDKNQSSSKRKAVVPGPAEHPLQYNYTFWYSRRTPGRPTSSQSYEQNIKQIGTFASVEQFWRFYSHMVRPGDLTGHSDFHLFKEGIKPMWEDDANKNGGKWIIRLRKGLASRCWENLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPNTIMEYKTHTDSIKMPGRLGPQRLLFQNLWKPRLNVP

Specificity

EIF4E2 - eukaryotic translation initiation factor 4E member 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for EIF4E2 Antibody (4G10)

Western Blot: EIF4E2 Antibody (4G10) [H00009470-M01]

Western Blot: EIF4E2 Antibody (4G10) [H00009470-M01]

Western Blot: EIF4E2 Antibody (4G10) [H00009470-M01] - EIF4E2 monoclonal antibody (M01), clone 4G10 Analysis of EIF4E2 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: EIF4E2 Antibody (4G10) [H00009470-M01]

Immunocytochemistry/ Immunofluorescence: EIF4E2 Antibody (4G10) [H00009470-M01]

Immunocytochemistry/Immunofluorescence: EIF4E2 Antibody (4G10) [H00009470-M01] - Analysis of monoclonal antibody to EIF4E2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: EIF4E2 Antibody (4G10) [H00009470-M01]

Immunohistochemistry-Paraffin: EIF4E2 Antibody (4G10) [H00009470-M01]

Immunohistochemistry-Paraffin: EIF4E2 Antibody (4G10) [H00009470-M01] - Analysis of monoclonal antibody to EIF4E2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.

Applications for EIF4E2 Antibody (4G10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

3 ug/ml

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EIF4E2

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures

Alternate Names

4EHP, 4E-LP, eIF4E type 2, eIF-4E type 2, EIF4EL3, eIF4E-like cap-binding protein, eIF4E-like protein 4E-LP, eukaryotic translation initiation factor 4E family member 2, Eukaryotic translation initiation factor 4E homologous protein, eukaryotic translation initiation factor 4E member 2, eukaryotic translation initiation factor 4E type 2, Eukaryotic translation initiation factor 4E-like 34E-LP, IF4e, mRNA cap-binding protein 4EHP, mRNA cap-binding protein type 3

Entrez Gene IDs

9470 (Human)

Gene Symbol

EIF4E2

OMIM

605895 (Human)

UniProt

AAH05392 (Human)

Additional EIF4E2 Products

Product Documents for EIF4E2 Antibody (4G10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EIF4E2 Antibody (4G10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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