EIF4E2 Antibody (OTI1F11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03611
![Flow Cytometry: EIF4E2 Antibody (OTI1F11) [NBP2-03611]](https://resources.bio-techne.com/images/products/EIF4E2-Antibody-OTI1F11-Flow-Cytometry-NBP2-03611-img0001.jpg "Flow Cytometry: EIF4E2 Antibody (OTI1F11) [NBP2-03611]")
![Western Blot: EIF4E2 Antibody (OTI1F11) [NBP2-03611]](https://resources.bio-techne.com/images/products/EIF4E2-Antibody-OTI1F11-Western-Blot-NBP2-03611-img0008.jpg "Western Blot: EIF4E2 Antibody (OTI1F11) [NBP2-03611]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1F11
Concentration
0.54 mg/ml
Product Summary for EIF4E2 Antibody (OTI1F11)
Immunogen
Full length human recombinant protein of human EIF4E2 (NP_004837) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
28.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for EIF4E2 Antibody (OTI1F11)
Western Blot: EIF4E2 Antibody (OTI1F11) [NBP2-03611]
Western Blot: EIF4E2 Antibody (OTI1F11) [NBP2-03611] - Analysis of extracts (10ug) from 1 cell lineby using anti-EIF4E2 monoclonal antibody.(1:200)![Immunocytochemistry/ Immunofluorescence: EIF4E2 Antibody (OTI1F11) [NBP2-03611]](https://resources.bio-techne.com/images/products/EIF4E2-Antibody-OTI1F11-Immunocytochemistry-Immunofluorescence-NBP2-03611-img0007.jpg "Immunocytochemistry/ Immunofluorescence: EIF4E2 Antibody (OTI1F11) [NBP2-03611]")
Immunocytochemistry/ Immunofluorescence: EIF4E2 Antibody (OTI1F11) [NBP2-03611]
Immunocytochemistry/Immunofluorescence: EIF4E2 Antibody (OTI1F11) [NBP2-03611] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF4E2.![Immunohistochemistry-Paraffin: EIF4E2 Antibody (OTI1F11) [NBP2-03611]](https://resources.bio-techne.com/images/products/EIF4E2-Antibody-OTI1F11-Immunohistochemistry-Paraffin-NBP2-03611-img0006.jpg "Immunohistochemistry-Paraffin: EIF4E2 Antibody (OTI1F11) [NBP2-03611]")
Immunohistochemistry-Paraffin: EIF4E2 Antibody (OTI1F11) [NBP2-03611]
Immunohistochemistry-Paraffin: EIF4E2 Antibody (OTI1F11) [NBP2-03611] - Staining of paraffin-embedded Human thyroid tissue using anti-EIF4E2 mouse monoclonal antibody.![Western Blot: EIF4E2 Antibody (OTI1F11) [NBP2-03611]](https://resources.bio-techne.com/images/products/EIF4E2-Antibody-OTI1F11-Western-Blot-NBP2-03611-img0002.jpg "Western Blot: EIF4E2 Antibody (OTI1F11) [NBP2-03611]")
![Immunohistochemistry-Paraffin: EIF4E2 Antibody (OTI1F11) [NBP2-03611]](https://resources.bio-techne.com/images/products/EIF4E2-Antibody-OTI1F11-Immunohistochemistry-Paraffin-NBP2-03611-img0003.jpg "Immunohistochemistry-Paraffin: EIF4E2 Antibody (OTI1F11) [NBP2-03611]")
![Immunohistochemistry-Paraffin: EIF4E2 Antibody (OTI1F11) [NBP2-03611]](https://resources.bio-techne.com/images/products/EIF4E2-Antibody-OTI1F11-Immunohistochemistry-Paraffin-NBP2-03611-img0004.jpg "Immunohistochemistry-Paraffin: EIF4E2 Antibody (OTI1F11) [NBP2-03611]")
![Immunohistochemistry-Paraffin: EIF4E2 Antibody (OTI1F11) [NBP2-03611]](https://resources.bio-techne.com/images/products/EIF4E2-Antibody-OTI1F11-Immunohistochemistry-Paraffin-NBP2-03611-img0005.jpg "Immunohistochemistry-Paraffin: EIF4E2 Antibody (OTI1F11) [NBP2-03611]")
Applications for EIF4E2 Antibody (OTI1F11)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.54 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: EIF4E2
Alternate Names
4EHP, 4E-LP, eIF4E type 2, eIF-4E type 2, EIF4EL3, eIF4E-like cap-binding protein, eIF4E-like protein 4E-LP, eukaryotic translation initiation factor 4E family member 2, Eukaryotic translation initiation factor 4E homologous protein, eukaryotic translation initiation factor 4E member 2, eukaryotic translation initiation factor 4E type 2, Eukaryotic translation initiation factor 4E-like 34E-LP, IF4e, mRNA cap-binding protein 4EHP, mRNA cap-binding protein type 3
Entrez Gene IDs
9470 (Human)
Gene Symbol
EIF4E2
Additional EIF4E2 Products
Product Documents for EIF4E2 Antibody (OTI1F11)
Product Specific Notices for EIF4E2 Antibody (OTI1F11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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