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EIF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16311

Novus Biologicals, part of Bio-Techne
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NBP2-16311

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (96%), Chicken (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF5. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EIF5 Antibody

Western Blot: EIF5 Antibody [NBP2-16311]

Western Blot: EIF5 Antibody [NBP2-16311]

Western Blot: EIF5 Antibody [NBP2-16311] - EIF5 antibody detects EIF5 protein by Western blot analysis. A. 50 ug mouse brain lysate/extract 10 % SDS-PAGE EIF5 antibody dilution: 1:1000
Immunohistochemistry-Paraffin: EIF5 Antibody [NBP2-16311]

Immunohistochemistry-Paraffin: EIF5 Antibody [NBP2-16311]

Immunohistochemistry-Paraffin: EIF5 Antibody [NBP2-16311] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Western Blot: EIF5 Antibody [NBP2-16311]

Western Blot: EIF5 Antibody [NBP2-16311]

Western Blot: EIF5 Antibody [NBP2-16311] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.

Applications for EIF5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-16311 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EIF5

Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM]

Alternate Names

eIF-5, EIF-5A, eukaryotic translation initiation factor 5

Gene Symbol

EIF5

UniProt

Additional EIF5 Products

Product Documents for EIF5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EIF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.