eIF5A Antibody (4D1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59403

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4D1

Format

BSA Free

Concentration

1 mg/ml

View all eIF5A Antibodies »

Product Specifications

Immunogen

Recombinant human eIF5A (1-154aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for eIF5A Antibody (4D1) - BSA Free

Western Blot: eIF5A Antibody (4D1)BSA Free [NBP2-59403]

Western Blot: eIF5A Antibody (4D1)BSA Free [NBP2-59403]

Western Blot: eIF5A Antibody (4D1) [NBP2-59403] - Lane 1: Jurkat cell lysates, Lane 2: 293T cell lysates, Lane 3: HeLa cell lysates, Lane 4: MCF7 cell lysates, Lane 5: NIH-3T3 cell lysates
Immunocytochemistry/ Immunofluorescence: eIF5A Antibody (4D1) - BSA Free [NBP2-59403]

Immunocytochemistry/ Immunofluorescence: eIF5A Antibody (4D1) - BSA Free [NBP2-59403]

Immunocytochemistry/Immunofluorescence: eIF5A Antibody (4D1) [NBP2-59403] - Analysis of EIF5A in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EIF5A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)
Flow Cytometry: eIF5A Antibody (4D1) - BSA Free [NBP2-59403]

Flow Cytometry: eIF5A Antibody (4D1) - BSA Free [NBP2-59403]

Flow Cytometry: eIF5A Antibody (4D1) [NBP2-59403] - Analysis of EIF5A in Balb/3T3 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line)

Applications for eIF5A Antibody (4D1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1015

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF5A

The human eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein. eIF5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post translational reactions. eIF5A promotes the formation of the first peptide bond during the initial stage of protein synthesis.

Long Name

Eukaryotic Translation Initiation Factor 5A

Alternate Names

eIF4D, Rev-binding Factor

Gene Symbol

EIF5A

Additional eIF5A Products

Product Documents for eIF5A Antibody (4D1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF5A Antibody (4D1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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