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eIF5A2 Antibody (1E7)

Novus Biologicals, part of Bio-Techne | Catalog # H00056648-M01

Novus Biologicals, part of Bio-Techne
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H00056648-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

EIF5A2 (NP_065123, 66 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GKKYEDICPSTHNMDVPNIKRNDYQLICIQDGYLSLLTETGEVREDLKLPEGELGKEIEGKYNAGEDVQVSVMCAMSEEYAVAIKPCK

Reactivity Notes

Human. Other species not tested.

Specificity

EIF5A2 - eukaryotic translation initiation factor 5A2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for eIF5A2 Antibody (1E7)

Western Blot: eIF5A2 Antibody (1E7) [H00056648-M01]

Western Blot: eIF5A2 Antibody (1E7) [H00056648-M01]

Western Blot: eIF5A2 Antibody (1E7) [H00056648-M01] - Analysis of EIF5A2 expression in transfected 293T cell line by EIF5A2 monoclonal antibody (M01), clone 1E7.Lane 1: EIF5A2 transfected lysate(16.8 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: eIF5A2 Antibody (1E7) [H00056648-M01] - Detection limit for recombinant GST tagged EIF5A2 is approximately 0.1ng/ml as a capture antibody.

Applications for eIF5A2 Antibody (1E7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF5A2

Eukaryotic translation initiation factor 5A (eIF5A) is an essential protein tightly linked to cellular polyamine homeostasis. EIF5A2, a novel phylogenetically conserved gene for eIF5A islocalized in an area of the genome often noted for chromosomal instability in cancers. EIF5A2 expression has been observed in testis, brain and colorectal adenocarcinoma

Alternate Names

EIF-5A2, eIF-5A-2, eIF5AII, eukaryotic initiation factor 5A, Eukaryotic initiation factor 5A isoform 2, eukaryotic translation initiation factor 5A2, eukaryotic translation initiation factor 5A-2

Entrez Gene IDs

56648 (Human)

Gene Symbol

EIF5A2

OMIM

605782 (Human)

Additional eIF5A2 Products

Product Documents for eIF5A2 Antibody (1E7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF5A2 Antibody (1E7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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