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eIF5A2 Antibody (OTI6G7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45697

Novus Biologicals, part of Bio-Techne
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NBP2-45697

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6G7

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human EIF5A2(NP_065123) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

16.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF5A2 Antibody (OTI6G7)

Western Blot: eIF5A2 Antibody (OTI6G7) [NBP2-45697]

Western Blot: eIF5A2 Antibody (OTI6G7) [NBP2-45697]

Western Blot: eIF5A2 Antibody (OTI6G7) [NBP2-45697] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF5A2.
Immunohistochemistry: eIF5A2 Antibody (OTI6G7) [NBP2-45697]

Immunohistochemistry: eIF5A2 Antibody (OTI6G7) [NBP2-45697]

Immunohistochemistry: eIF5A2 Antibody (OTI6G7) [NBP2-45697] - Analysis of Human pancreas tissue.
Flow Cytometry: eIF5A2 Antibody (OTI6G7) [NBP2-45697]

Flow Cytometry: eIF5A2 Antibody (OTI6G7) [NBP2-45697]

Flow Cytometry: eIF5A2 Antibody (OTI6G7) [NBP2-45697] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by eIF5A2 antibody.

Applications for eIF5A2 Antibody (OTI6G7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF5A2

Eukaryotic translation initiation factor 5A (eIF5A) is an essential protein tightly linked to cellular polyamine homeostasis. EIF5A2, a novel phylogenetically conserved gene for eIF5A islocalized in an area of the genome often noted for chromosomal instability in cancers. EIF5A2 expression has been observed in testis, brain and colorectal adenocarcinoma

Alternate Names

EIF-5A2, eIF-5A-2, eIF5AII, eukaryotic initiation factor 5A, Eukaryotic initiation factor 5A isoform 2, eukaryotic translation initiation factor 5A2, eukaryotic translation initiation factor 5A-2

Gene Symbol

EIF5A2

Additional eIF5A2 Products

Product Documents for eIF5A2 Antibody (OTI6G7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF5A2 Antibody (OTI6G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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