Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CELA3B/1257

Concentration

0.2 mg/ml

View all Elastase Antibodies »

Product Specifications

Immunogen

Recombinant human Elastase protein fragment (around aa 82-238) (exact sequence is proprietary) (Uniprot: P08861)

Localization

Cytoplasmic

Marker

Pancreatic Function Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54301)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Elastase Antibody (CELA3B/1257)

Western Blot: Elastase Antibody (CELA3B/1257) [NBP2-53246]

Western Blot: Elastase Antibody (CELA3B/1257) [NBP2-53246]

Western Blot: Elastase Antibody (CELA3B/1257) [NBP2-53246] - Western Blot Analysis of PANC-1 cell lysate using Elastase antibody (CELA3B/1257).
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-53246]

Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-53246]

Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-53246] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-53246]

Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-53246]

Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-53246] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257)
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Applications for Elastase Antibody (CELA3B/1257)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Elastase

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have sixelastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike otherelastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted fromthe pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has adigestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3Bmay also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B havebeen referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently usedas a measure of pancreatic function in clinical assays. (provided by RefSeq)

Alternate Names

CBPP, cholesterol-binding pancreatic protease, chymotrypsin-like elastase family member 3B, chymotrypsin-like elastase family, member 3B, E1, EC 3.4.21, EC 3.4.21.70, EL-1, ELA3B, elastase 1, elastase 3B, pancreatic, Elastase IIIB, elastase-3B, fecal elastase 1, pancreatic elastase 1, pancreatic endopeptidase E, Protease E, proteinase E

Gene Symbol

CELA3B

UniProt

P08861 (Human)

Additional Elastase Products

Product Documents for Elastase Antibody (CELA3B/1257)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Elastase Antibody (CELA3B/1257)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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