Elastase Antibody (CELA3B/1811)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53284
![Flow Cytometry: Elastase Antibody (CELA3B/1811) [NBP2-53284]](https://resources.bio-techne.com/images/products/Elastase-Antibody-CELA3B-1811-Flow-Cytometry-NBP2-53284-img0002.jpg "Flow Cytometry: Elastase Antibody (CELA3B/1811) [NBP2-53284]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CELA3B/1811
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human Elastase protein fragment (around aa 82-238) (exact sequence is proprietary) (Uniprot: P08861)
Localization
Cytoplasmic
Marker
Pancreatic Function Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54489)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Elastase Antibody (CELA3B/1811)
Flow Cytometry: Elastase Antibody (CELA3B/1811) [NBP2-53284]
Flow Cytometry: Elastase Antibody (CELA3B/1811) [NBP2-53284] - Human bronchoalveolar lavage fluid. CF BALF cells stained for analysis by flow cytometry, gated on live neutrophils showing presence of NE on the cell surface. Flow cytometry image submitted by a verified customer review. Image from the Alexa Fluor 488 version of this antibody.Applications for Elastase Antibody (CELA3B/1811)
Application
Recommended Usage
ELISA
2 - 4 ug/ml
Flow Cytometry
0.5 - 1 ug/million cells
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Elastase
Alternate Names
CBPP, cholesterol-binding pancreatic protease, chymotrypsin-like elastase family member 3B, chymotrypsin-like elastase family, member 3B, E1, EC 3.4.21, EC 3.4.21.70, EL-1, ELA3B, elastase 1, elastase 3B, pancreatic, Elastase IIIB, elastase-3B, fecal elastase 1, pancreatic elastase 1, pancreatic endopeptidase E, Protease E, proteinase E
Gene Symbol
CELA3B
UniProt
Additional Elastase Products
Product Documents for Elastase Antibody (CELA3B/1811)
Product Specific Notices for Elastase Antibody (CELA3B/1811)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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