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ELF5 Antibody (3D10)

Novus Biologicals, part of Bio-Techne | Catalog # H00002001-M01

Novus Biologicals, part of Bio-Techne
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H00002001-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3D10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ELF5 (NP_938195.1, 166 a.a. ~ 263 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SRTSLQSSHLWEFVRDLLLSPEENCGILEWEDREQGIFRVVKSEALAKMWGQRKKNDRMTYEKLSRALRYYYKTGILERVDRRLVYKFGKNAHGWQED

Specificity

ELF5 - E74-like factor 5 (ets domain transcription factor) (3D10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ELF5 Antibody (3D10)

Western Blot: ELF5 Antibody (3D10) [H00002001-M01]

Western Blot: ELF5 Antibody (3D10) [H00002001-M01]

Western Blot: ELF5 Antibody (3D10) [H00002001-M01] - Analysis of ELF5 expression in transfected 293T cell line by ELF5 monoclonal antibody (M01), clone 3D10.Lane 1: ELF5 transfected lysate(30.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: ELF5 Antibody (3D10) [H00002001-M01] - Detection limit for recombinant GST tagged ELF5 is 3 ng/ml as a capture antibody.

Applications for ELF5 Antibody (3D10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ELF5

The protein encoded by this gene is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Alternate Names

E74-like factor 5, E74-like factor 5 (ets domain transcription factor), Epithelium-restricted ESE-1-related Ets factor, Epithelium-specific Ets transcription factor 2, ESE2ESE-2, ETS-related transcription factor Elf-5

Entrez Gene IDs

2001 (Human)

Gene Symbol

ELF5

OMIM

605169 (Human)

UniProt

Additional ELF5 Products

Product Documents for ELF5 Antibody (3D10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ELF5 Antibody (3D10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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