Elk-1 Antibody (3H6D12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47553

Novus Biologicals, part of Bio-Techne
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NBP1-47553
NBP1-47553-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3H6D12

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Elk-1 Antibodies »

Product Summary for Elk-1 Antibody (3H6D12) - BSA Free

Immunogen

Purified recombinant fragment of Elk-1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Elk-1 Antibody (3H6D12) - BSA Free

Western Blot: Elk-1 Antibody (3H6D12)BSA Free [NBP1-47553]

Western Blot: Elk-1 Antibody (3H6D12)BSA Free [NBP1-47553]

Western Blot: Elk-1 Antibody (3H6D12) [NBP1-47553] - Analysis using ELK1 mouse mAb against truncated ELK1 recombinant protein (1) and K562 cell lysate (2).
Immunohistochemistry-Paraffin: Elk-1 Antibody (3H6D12) - BSA Free [NBP1-47553]

Immunohistochemistry-Paraffin: Elk-1 Antibody (3H6D12) - BSA Free [NBP1-47553]

Immunohistochemistry-Paraffin: Elk-1 Antibody (3H6D12) [NBP1-47553] - Analysis of human brain tumor tissue, showing nuclear and cytoplasmic localization using ELK1 mouse mAb with DAB staining.

Applications for Elk-1 Antibody (3H6D12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Elk-1

The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.

Alternate Names

Elk1

Entrez Gene IDs

2002 (Human)

Gene Symbol

ELK1

UniProt

P19419 (Human)

Additional Elk-1 Products

Product Documents for Elk-1 Antibody (3H6D12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Elk-1 Antibody (3H6D12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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