Elk-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38278

Novus Biologicals, part of Bio-Techne
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NBP2-38278
NBP2-38278-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Elk-1 Antibodies »

Product Summary for Elk-1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HPRPAVVLPSAAPAGAAAPPSGSRSTSPSPLEACLEAEEAGLPLQVILTPPEAPNLKSEELNVEPGLGRALPPEVKVEGPKEE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Elk-1 Antibody

Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278]

Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278]

Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278] - Staining in human ovary and pancreas tissues using anti-ELK1 antibody. Corresponding ELK1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Elk-1 Antibody [NBP2-38278]

Immunocytochemistry/ Immunofluorescence: Elk-1 Antibody [NBP2-38278]

Immunocytochemistry/Immunofluorescence: Elk-1 Antibody [NBP2-38278] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278]

Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278]

Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278] - Staining of human pancreas shows low expression as expected.
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Applications for Elk-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Elk-1

ELK is a member of the family of genes homologous to the v-Ets oncogene isolated from the E26 eythroblastosis virus. Members of this family exhibit varied patterns of tissue expression. They also share a highly conserved C-terminal domain that may be responsible for the DNA binding activity of all members of the Ets gene family.

Alternate Names

Elk1

Gene Symbol

ELK1

UniProt

P19419 (Human)

Additional Elk-1 Products

Product Documents for Elk-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Elk-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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