ELK3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16315

Novus Biologicals, part of Bio-Techne
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NBP2-16315
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Bovine (91%), Rat (92%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Chemotaxis, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ELK3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ELK3. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (88%). Mouse reactivity reported in scientific literature (PMID: 26590320).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ELK3 Antibody

Western Blot: ELK3 Antibody [NBP2-16315]

Western Blot: ELK3 Antibody [NBP2-16315]

Western Blot: ELK3 Antibody [NBP2-16315] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ELK3 Antibody [NBP2-16315]

Immunocytochemistry/ Immunofluorescence: ELK3 Antibody [NBP2-16315]

Immunocytochemistry/Immunofluorescence: ELK3 Antibody [NBP2-16315] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ELK3 protein stained by ELK3 antibody [N1C2] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: ELK3 Antibody [NBP2-16315]

Immunohistochemistry-Paraffin: ELK3 Antibody [NBP2-16315]

Immunohistochemistry-Paraffin: ELK3 Antibody [NBP2-16315] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Applications for ELK3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IHC-Fr usage reported in scientific literature (PMID: 26590320).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ELK3

The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. [provided by RefSeq]

Alternate Names

ELK3, ETS-domain protein (SRF accessory protein 2), ERPETS domain-containing protein Elk-3, ETS-domain protein, ETS-related protein ERP, NETSRF accessory protein 2, SAP-2, SAP2ETS-related protein NET, Serum response factor accessory protein 2

Gene Symbol

ELK3

UniProt

P41970 (Human)

Additional ELK3 Products

Product Documents for ELK3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ELK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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