ELMO1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-875
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence PKEPSNYDFVYDCN corresponding to C-Terminus according to NP_055615.8, NP_569709.1, NP_001034548.1.
Specificity
This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for ELMO1 Antibody
![Western Blot: ELMO1 Antibody [NB100-875]](https://resources.bio-techne.com/images/products/ELMO1-Antibody-Western-Blot-NB100-875-img0002.jpg "Western Blot: ELMO1 Antibody [NB100-875]")
Western Blot: ELMO1 Antibody [NB100-875]
Western Blot: ELMO1 Antibody [NB100-875] - (0.3ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunoprecipitation: ELMO1 Antibody [NB100-875]](https://resources.bio-techne.com/images/products/ELMO1-Antibody-Immunoprecipitation-NB100-875-img0003.jpg "Immunoprecipitation: ELMO1 Antibody [NB100-875]")
Immunoprecipitation: ELMO1 Antibody [NB100-875]
Immunoprecipitation: ELMO1 Antibody [NB100-875] - (0.3ug/ml) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for ELMO1 Antibody
Application
Recommended Usage
Immunoprecipitation
1:10 - 1:500
Peptide ELISA
Detection limit 1:128000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
Western blot: Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8).
Immunoprecipitation: This antibody has been successfully used in IP from BAEC cells as described in the following paper: Epting et al, Circ Res. 2010 Jul 9;107(1):45-55; PMID: 20466982. IP; An anonymous customer found positive results in IP on Human promyelocytic leukemia cells (HL60)
Immunoprecipitation: This antibody has been successfully used in IP from BAEC cells as described in the following paper: Epting et al, Circ Res. 2010 Jul 9;107(1):45-55; PMID: 20466982. IP; An anonymous customer found positive results in IP on Human promyelocytic leukemia cells (HL60)
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ELMO1
Alternate Names
ced-12 homolog 1, CED12CED-12, ELMO-1, engulfment and cell motility 1, engulfment and cell motility 1 (ced-12 homolog, C. elegans), engulfment and cell motility protein 1, KIAA0281MGC126406, Protein ced-12 homolog
Gene Symbol
ELMO1
UniProt
Additional ELMO1 Products
Product Documents for ELMO1 Antibody
Product Specific Notices for ELMO1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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