ELMO3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-873

Novus Biologicals, part of Bio-Techne
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NB100-873
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ELMO3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence TNFNFCYDCSIAEP corresponding to C-Terminus according to NP_078988.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ELMO3 Antibody

Western Blot: ELMO3 Antibody [NB100-873]

Western Blot: ELMO3 Antibody [NB100-873]

Western Blot: ELMO3 Antibody [NB100-873] - Western blot analysis of ELMO3 in Human Brain lysate using NB100-873 at 2 ug/ ml.
Western Blot: ELMO3 Antibody [NB100-873]

Western Blot: ELMO3 Antibody [NB100-873]

Western Blot: ELMO3 Antibody [NB100-873] - (0.1ug/ml) of Human Brain (Hipoocampus) lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Western Blot: ELMO3 Antibody [NB100-873]

Western Blot: ELMO3 Antibody [NB100-873]

Western Blot: ELMO3 Antibody [NB100-873] - Staining (2ug/ml) of Mouse Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for ELMO3 Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in human brain (hippocampus) lysates (calculated MW of 70.0 kDa band according to NP_ 078988.2). IHC-P: Human prostate shows vesiculate staining in the cytoplasm of secretory cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ELMO3

The protein encoded by this gene is similar to a C. elegans protein that functions in phagocytosis of apoptotic cells and in cell migration. Other members of this small family of engulfment and cell motility (ELMO) proteins have been shown to interact with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes.

Alternate Names

CED12, CED-12, ced-12 homolog 3, ELMO-3, engulfment and cell motility 3, engulfment and cell motility 3 (ced-12 homolog, C. elegans), engulfment and cell motility protein 3, FLJ13824

Entrez Gene IDs

79767 (Human)

Gene Symbol

ELMO3

UniProt

NP_078988. (Human)

Additional ELMO3 Products

Product Documents for ELMO3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ELMO3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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