Elongin A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87040

Novus Biologicals, part of Bio-Techne
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NBP1-87040
NBP1-87040-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Elongin A Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ERHLGEPHGKGVVSQNKEHKSSHKDKRPVDAKSDEKASVVSREKSHKALSKEENRRPPSGDNAREKPPSSGVKKEKDREGSSLKKKCLPPSEAASDNHLKKPKHRDPEKAKLDKSKQGLDSFDTGKGAGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Elongin A Antibody

Western Blot: Elongin A Antibody [NBP1-87040]

Western Blot: Elongin A Antibody [NBP1-87040]

Western Blot: Elongin A Antibody [NBP1-87040] - Analysis in human cell line U-2197.
Immunocytochemistry/ Immunofluorescence: Elongin A Antibody [NBP1-87040]

Immunocytochemistry/ Immunofluorescence: Elongin A Antibody [NBP1-87040]

Immunocytochemistry/Immunofluorescence: Elongin A Antibody [NBP1-87040] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040]

Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040]

Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040] - Staining of human liver shows moderate to strong nuclear positivity in hepatocytes.
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Applications for Elongin A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Elongin A

TCEB3 (Elongin A) is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Alternate Names

EloA, Elongin 110 kDa subunit, elongin-A, FLJ38760, FLJ42849, RNA polymerase II transcription factor SIII subunit A1, SIII, SIII p110, TCEB3A, transcription elongation factor B (SIII), polypeptide 3 (110kD, elongin A), transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A), transcription elongation factor B alpha subunit, transcription elongation factor B polypeptide 3

Gene Symbol

TCEB3

Additional Elongin A Products

Product Documents for Elongin A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Elongin A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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