ELOVL5 Antibody (JE65-70)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32305
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE65-70
Concentration
1 mg/ml
Product Summary for ELOVL5 Antibody (JE65-70)
Immunogen
Synthetic peptide within human ELOVL5 aa 250-299/299. (Uniprot: Q9NYP7)
Localization
Endoplasmic reticulum membrane, dendrite.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ELOVL5 Antibody (JE65-70)
Western Blot: ELOVL5 Antibody (JE65-70) [NBP3-32305]
Western Blot: ELOVL5 Antibody (JE65-70) [NBP3-32305] - Western blot analysis of ELOVL5 on different lysates with Rabbit anti-ELOVL5 antibody (NBP3-32305) at 1/500 dilution.Lane 1: Rat kidney tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 35 kDa
Observed band size: 38 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32305) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Applications for ELOVL5 Antibody (JE65-70)
Application
Recommended Usage
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ELOVL5
Alternate Names
dJ483K16.1, EC 2.3.1.n8, elongation of very long chain fatty acids protein 5, ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, ELOVL2,3-keto acyl-CoA synthase ELOVL5, Fatty acid elongase 1, hELO1, HELO1homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2, homolog of yeast long chain polyunsaturated fatty acid elongatio, SUR4/Elo3-like, yeast)
Gene Symbol
ELOVL5
Additional ELOVL5 Products
Product Documents for ELOVL5 Antibody (JE65-70)
Product Specific Notices for ELOVL5 Antibody (JE65-70)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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