ELOVL5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33500

Novus Biologicals, part of Bio-Techne
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NBP2-33500
NBP2-33500-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ELOVL5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: QTYNKKGASRRKDHLKDHQNGSMAAVNGHTNSFSPLENNVKPRKLRKD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ELOVL5 Antibody

Western Blot: ELOVL5 Antibody [NBP2-33500]

Western Blot: ELOVL5 Antibody [NBP2-33500]

Western Blot: ELOVL5 Antibody [NBP2-33500] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ELOVL5 Antibody [NBP2-33500]

Immunocytochemistry/ Immunofluorescence: ELOVL5 Antibody [NBP2-33500]

Immunocytochemistry/Immunofluorescence: ELOVL5 Antibody [NBP2-33500] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ELOVL5 Antibody [NBP2-33500]

Immunohistochemistry-Paraffin: ELOVL5 Antibody [NBP2-33500]

Immunohistochemistry-Paraffin: ELOVL5 Antibody [NBP2-33500] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
View 4 more images

Applications for ELOVL5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 -1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ELOVL5

ELOVL5 plays a role in elongation of long-chain polyunsaturated fatty acids (Leonard et al., 2000)

Alternate Names

dJ483K16.1, EC 2.3.1.n8, elongation of very long chain fatty acids protein 5, ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, ELOVL2,3-keto acyl-CoA synthase ELOVL5, Fatty acid elongase 1, hELO1, HELO1homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2, homolog of yeast long chain polyunsaturated fatty acid elongatio, SUR4/Elo3-like, yeast)

Gene Symbol

ELOVL5

UniProt

Q9NYP7 (Human)

Additional ELOVL5 Products

Product Documents for ELOVL5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ELOVL5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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