Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Elp4 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 374 and 424 of human Elongation Protein 4 using the numbering given in entry NP_061913.3 (GeneID 26610).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Elp4 Antibody

Immunoprecipitation: Elp4 Antibody [NBP2-12785]

Immunoprecipitation: Elp4 Antibody [NBP2-12785]

Immunoprecipitation: Elp4 Antibody [NBP2-12785] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ELP4 antibody NBP2-12785 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ELP4 was also immunoprecipitated by rabbit anti-ELP4 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated ELP4. Detection:Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Applications for Elp4 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Elp4

Elp4 encodes a component of the six subunit elongator complex, a histone acetyltransferase complex that associates directly with RNA polymerase II during transcriptional elongation. The human gene can partially complement sensitivity phenotypes of yeast ELP4 deletion mutants. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined.

Alternate Names

C11orf19, chromosome 11 open reading frame 19, dJ68P15A.1, elongation protein 4 homolog (S. cerevisiae), elongator complex protein 4, hELP4, PAX6 neighbor gene protein, PAX6NEB, PAXNEBFLJ20498

Gene Symbol

ELP4

UniProt

NP_061913.3 (Human)

Additional Elp4 Products

Product Documents for Elp4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Elp4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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