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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunoprecipitation, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A syntetic peptide between aa2220 and C-term

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ELYS Antibody

Immunoprecipitation: ELYS Antibody [NB600-238]

Immunoprecipitation: ELYS Antibody [NB600-238]

Immunoprecipitation: ELYS Antibody [NB600-238] - Nuclear Extract (10 and 20 ug for WB; 1 mg for IP/WB) from HeLa cells. Antibodies: A) Affinity purified rabbit anti-ELYS antibody BL476 used at 0.5 ug/ml for WB and 4 ug/mg lysate for IP. B) Affinity purified rabbit anti-ELYS antibodies BL476 and BL475 were each used at 4 ug/mg extract for IP. Immunoprecipitated ELYS was detected by WB using BL475 at 0.2 ug/ml. Detection: Chemiluminescence with exposure times of 3 to 5 minutes.

Applications for ELYS Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in Simple Western reported in scientific literature (PMID:35017527) By Western Blot, this antibody recognizes a band at ~252.5 kDa, representing ELYS protein. May be used for immunoprecipitation, as well. Western blot of immunoprecipitates was performed using an immunoprecipitation/Western blot reagent and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ELYS

ELYS is a novel nuclear transcription factor expressed in embryonic haematopoietic tissues and plays a role in both early and mature haematopoietic events.

Alternate Names

AT hook containing transcription factor 1, ELYS, ELYS transcription factor like protein TMBS62, ELYS transcription factor-like protein TMBS62, MST108, MSTP108, protein ELYS, putative AT-hook-containing transcription factor 1, TMBS62

Entrez Gene IDs

25909 (Human)

Gene Symbol

AHCTF1

Additional ELYS Products

Product Documents for ELYS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ELYS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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