Emerin Antibody (8F5A8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52459
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 8F5A8
Format
BSA Free
Concentration
1 mg/ml
Product Summary for Emerin Antibody (8F5A8) - BSA Free
Immunogen
Purified recombinant fragment of human Emerin (AA: 1-222) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Emerin Antibody (8F5A8) - BSA Free
Western Blot: Emerin Antibody (8F5A8)BSA Free [NBP2-52459]
Western Blot: Emerin Antibody (8F5A8) [NBP2-52459] - Analysis using EMD mAb against human EMD (AA: 1-222) recombinant protein. (Expected MW is 51.1 kDa)Immunocytochemistry/ Immunofluorescence: Emerin Antibody (8F5A8) - BSA Free [NBP2-52459]
Immunocytochemistry/Immunofluorescence: Emerin Antibody (8F5A8) [NBP2-52459] - Analysis of Hela cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Immunohistochemistry: Emerin Antibody (8F5A8) - BSA Free [NBP2-52459]
Immunohistochemistry: Emerin Antibody (8F5A8) [NBP2-52459] - Analysis of paraffin-embedded ovarian cancer tissues using EMD mouse mAb with DAB staining.Applications for Emerin Antibody (8F5A8) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Emerin
Alternate Names
emerin, Emery-Dreifuss muscular dystrophy, LEM domain containing 5, STAEDMDLEMD5
Gene Symbol
EMD
Additional Emerin Products
Product Documents for Emerin Antibody (8F5A8) - BSA Free
Product Specific Notices for Emerin Antibody (8F5A8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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