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EMG1 Antibody (OTI1B8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02575

Novus Biologicals, part of Bio-Techne
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NBP2-02575

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1B8

Concentration

0.97 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human EMG1(NP_006322) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

26.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EMG1 Antibody (OTI1B8)

Western Blot: EMG1 Antibody (1B8) [NBP2-02575] Analysis of extracts (35ug) from 9 different cell lines by using anti-EMG1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: EMG1 Antibody (OTI1B8) [NBP2-02575]

Immunocytochemistry/ Immunofluorescence: EMG1 Antibody (OTI1B8) [NBP2-02575]

Immunocytochemistry/Immunofluorescence: EMG1 Antibody (1B8) [NBP2-02575] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EMG1.
Flow Cytometry: EMG1 Antibody (OTI1B8) [NBP2-02575]

Flow Cytometry: EMG1 Antibody (OTI1B8) [NBP2-02575]

Flow Cytometry: EMG1 Antibody (1B8) [NBP2-02575] - Analysis of Jurkat cells, using anti-EMG1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for EMG1 Antibody (OTI1B8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.97 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EMG1

This gene encodes an essential, conserved eukaryotic protein involved in ribosome biogenesis. In yeast, the related protein is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome. COMPLETENESS: complete on the 3' end.

Alternate Names

18S rRNA (pseudouridine-N1-)-methyltransferase NEP1, BWCNS, C2F18S rRNA Psi1248 methyltransferase, EMG1 nucleolar protein homolog (S. cerevisiae), essential for mitotic growth 1, FLJ60792, Grcc2f, NEP1EC 2.1.1.-, Nucleolar protein EMG1 homolog, probable ribosome biogenesis protein NEP1, Protein C2f, Ribosome biogenesis protein NEP1

Entrez Gene IDs

10436 (Human)

Gene Symbol

EMG1

Additional EMG1 Products

Product Documents for EMG1 Antibody (OTI1B8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EMG1 Antibody (OTI1B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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