EMI1 Antibody (EMI1/1176)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44344
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # EMI1/1176
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4)
Localization
Nuclear
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47786)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for EMI1 Antibody (EMI1/1176)
Western Blot: EMI1 Antibody (EMI1/1176) [NBP2-44344]
Western Blot: EMI1 Antibody (EMI1/1176) [NBP2-44344] - Western Blot of HeLa, HepG2, HEK293 and K562 cell lysates with EMI1 Antibody (EMI1/1176).Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) [NBP2-44344]
Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) [NBP2-44344] - Human Ovarian carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) [NBP2-44344]
Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) [NBP2-44344] - Human Ovarian Carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).Applications for EMI1 Antibody (EMI1/1176)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: EMI1
Alternate Names
EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31
Gene Symbol
FBXO5
UniProt
Additional EMI1 Products
Product Documents for EMI1 Antibody (EMI1/1176)
Product Specific Notices for EMI1 Antibody (EMI1/1176)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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