EML4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05973
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human echinoderm microtubule associated protein like 4 using the numbering given in entry NP_061936.2 (GeneID 27436).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EML4 Antibody
![Western Blot: EML4 Antibody [NBP1-05973]](https://resources.bio-techne.com/images/products/EML4-Antibody-Western-Blot-NBP1-05973-img0004.jpg "Western Blot: EML4 Antibody [NBP1-05973]")
Western Blot: EML4 Antibody [NBP1-05973]
Western Blot: EML4 Antibody [NBP1-05973] - Detection of Human EML4 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EML4 antibody NBP1-05973 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05973]](https://resources.bio-techne.com/images/products/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-05973-img0005.jpg "Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05973]")
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05973]
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05973] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-EML4 used at a dilution of1:1000 (0.2ug/ml). Detection: DAB![Immunoprecipitation: EML4 Antibody [NBP1-05973]](https://resources.bio-techne.com/images/products/EML4-Antibody-Immunoprecipitation-NBP1-05973-img0006.jpg "Immunoprecipitation: EML4 Antibody [NBP1-05973]")
Immunoprecipitation: EML4 Antibody [NBP1-05973]
Immunoprecipitation: EML4 Antibody [NBP1-05973] - Detection of human EML4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EML4 antibody NBP1-05973 (lot NBP1-05973-2) used for IP at 6 ug per reaction. EML4 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05973-1) and rabbit anti-EML4 antibody NBP1-05974. For blotting immunoprecipitated EML4, NBP1-05973 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for EML4 Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: EML4
Alternate Names
C2orf2, echinoderm microtubule associated protein like 4, echinoderm microtubule-associated protein-like 4, ELP120, EMAP-4, EMAPL4, FLJ10942, FLJ32318, Restrictedly overexpressed proliferation-associated protein, Ropp 120, ROPP120DKFZp686P18118
Entrez Gene IDs
27436 (Human)
Gene Symbol
EML4
UniProt
Additional EML4 Products
Product Documents for EML4 Antibody
Product Specific Notices for EML4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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