Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all EN-RAGE/S100A12 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human S100A12 (NP_005612) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

10.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EN-RAGE/S100A12 Antibody (OTI1D1) - Azide and BSA Free

Western Blot: EN-RAGE/S100A12 Antibody (OTI1D1)Azide and BSA Free [NBP2-71276]

Western Blot: EN-RAGE/S100A12 Antibody (OTI1D1)Azide and BSA Free [NBP2-71276]

Western Blot: EN-RAGE/S100A12 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71276] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A12.
Immunohistochemistry: EN-RAGE/S100A12 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71276]

Immunohistochemistry: EN-RAGE/S100A12 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71276]

Immunohistochemistry: EN-RAGE/S100A12 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71276] - Staining of paraffin-embedded Human liver tissue using anti-S100A12 mouse monoclonal antibody.
Immunohistochemistry: EN-RAGE/S100A12 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71276]

Immunohistochemistry: EN-RAGE/S100A12 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71276]

Immunohistochemistry: EN-RAGE/S100A12 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71276] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-S100A12 mouse monoclonal antibody.
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Applications for EN-RAGE/S100A12 Antibody (OTI1D1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EN-RAGE/S100A12

S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A12 is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. S100A12 is also a ligand for RAGE. Interaction with RAGE on mononuclear phagocytes, lymphocytes and endothelium triggers cellular activation. Nuclear factor kappa B--a key transcription factor for inflammatory events--is activated by S100A12/RAGE. S100A12 has also been shown to have anti-microbial properties.

Long Name

Extracellular Newly Identified RAGE-binding Protein

Alternate Names

CAAF1, CAAFI, CAGC, CAGCS100, Calgranulin C, ENRAGE, MRP6, S100A12

Gene Symbol

S100A12

Additional EN-RAGE/S100A12 Products

Product Documents for EN-RAGE/S100A12 Antibody (OTI1D1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EN-RAGE/S100A12 Antibody (OTI1D1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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