EN1/Engrailed 1 Antibody (3H2)

Novus Biologicals, part of Bio-Techne | Catalog # H00002019-M04

Novus Biologicals, part of Bio-Techne
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H00002019-M04
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3H2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Engrailed-1 Antibodies »

Product Specifications

Immunogen

EN1 (NP_001417, 266 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SQQPLVWPAWVYCTRYSDRPSSGPRTRKLKKKKNEKEDKRPRTAFTAEQLQRLKAEFQANRYITEQRRQTLAQELSLNESQIKIWFQNKRAKIKKATGIKNGLALHLMAQGLYNHSTTTVQDKDESE*

Specificity

EN1 (3H2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EN1/Engrailed 1 Antibody (3H2)

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04]

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04]

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04] - Analysis of monoclonal antibody to EN1 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml
ELISA: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04]

ELISA: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04]

ELISA: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04] - Detection limit for recombinant GST tagged EN1 is approximately 1ng/ml as a capture antibody.

Applications for EN1/Engrailed 1 Antibody (3H2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for IHC-P.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Engrailed-1

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Alternate Names

EN1, Engrailed1

Entrez Gene IDs

2019 (Human)

Gene Symbol

EN1

OMIM

131290 (Human)

UniProt

NP_001417 (Human)

Additional Engrailed-1 Products

Product Documents for EN1/Engrailed 1 Antibody (3H2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EN1/Engrailed 1 Antibody (3H2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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