Enah/Vasp-like Antibody (5G1)
Novus Biologicals, part of Bio-Techne | Catalog # H00051466-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5G1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
EVL (NP_057421, 309 a.a. ~ 418 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PGTRAASQPPNSSEAGRKPWERSNSVEKPVSSILSRTPSVAKSPEAKSPLQSQPHSRMKPAGSVNDMALDAFDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT
Reactivity Notes
Human. Other species not tested.
Localization
Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Note=Targeted to the leading edge of lamellipodia and the distal tip of stress fibers through interaction with a number of proteins. In activated T-cells, localizes to the F-actin collar and the dis
Specificity
EVL - Enah/Vasp-like
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Enah/Vasp-like Antibody (5G1)
![Western Blot: Enah/Vasp-like Antibody (5G1) [H00051466-M01]](https://resources.bio-techne.com/images/products/Enah-Vasp-like-Antibody-5G1-Western-Blot-H00051466-M01-img0007.jpg "Western Blot: Enah/Vasp-like Antibody (5G1) [H00051466-M01]")
Western Blot: Enah/Vasp-like Antibody (5G1) [H00051466-M01]
Western Blot: Enah/Vasp-like Antibody (5G1) [H00051466-M01] - Analysis of EVL expression in transfected 293T cell line by EVL monoclonal antibody (M01), clone 5G1.Lane 1: EVL transfected lysate(45 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: Enah/Vasp-like Antibody (5G1) [H00051466-M01]](https://resources.bio-techne.com/images/products/Enah-Vasp-like-Antibody-5G1-Immunocytochemistry-Immunofluorescence-H00051466-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Enah/Vasp-like Antibody (5G1) [H00051466-M01]")
Immunocytochemistry/ Immunofluorescence: Enah/Vasp-like Antibody (5G1) [H00051466-M01]
Immunocytochemistry/Immunofluorescence: Enah/Vasp-like Antibody (5G1) [H00051466-M01] - Analysis of monoclonal antibody to EVL on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Enah/Vasp-like Antibody (5G1) [H00051466-M01] - Detection limit for recombinant GST tagged EVL is approximately 0.03ng/ml as a capture antibody.
Applications for Enah/Vasp-like Antibody (5G1)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA immunofluorescence.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Enah/Vasp-like
Alternate Names
ena/VASP-like protein, Enah/Vasp-like, RNB6Ena/vasodilator-stimulated phosphoprotein-like
Entrez Gene IDs
51466 (Human)
Gene Symbol
EVL
UniProt
Additional Enah/Vasp-like Products
Product Documents for Enah/Vasp-like Antibody (5G1)
Product Specific Notices for Enah/Vasp-like Antibody (5G1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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