Endo G Antibody (JU31-30)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75480

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JU31-30

Concentration

1 mg/ml

View all Endo G Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Endo G aa 200-297 / 297. (SwissProt: Q14249 Human)

Localization

Mitochondrion. Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endo G Antibody (JU31-30)

Western Blot: Endo G Antibody (JU31-30) [NBP2-75480]

Western Blot: Endo G Antibody (JU31-30) [NBP2-75480]

Western Blot: Endo G Antibody (JU31-30) [NBP2-75480] - Analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: Endo G Antibody (JU31-30) [NBP2-75480]

Immunocytochemistry/ Immunofluorescence: Endo G Antibody (JU31-30) [NBP2-75480]

Immunocytochemistry/Immunofluorescence: Endo G Antibody (JU31-30) [NBP2-75480] - Staining Endo G in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised
Immunocytochemistry/ Immunofluorescence: Endo G Antibody (JU31-30) [NBP2-75480]

Immunocytochemistry/ Immunofluorescence: Endo G Antibody (JU31-30) [NBP2-75480]

Immunocytochemistry/Immunofluorescence: Endo G Antibody (JU31-30) [NBP2-75480] - Staining Endo G in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Applications for Endo G Antibody (JU31-30)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endo G

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).

Alternate Names

EC 3.1.30.-, Endo G, endonuclease G, endonuclease G, mitochondrial, FLJ27463, mitochondrial endonuclease G

Gene Symbol

ENDOG

Additional Endo G Products

Product Documents for Endo G Antibody (JU31-30)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Endo G Antibody (JU31-30)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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