Endo G Antibody (OTI5F1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01696

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5F1

Concentration

1 mg/ml

View all Endo G Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ENDOG(NP_004426) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endo G Antibody (OTI5F1)

Western Blot: Endo G Antibody (OTI5F1) [NBP2-01696]

Western Blot: Endo G Antibody (OTI5F1) [NBP2-01696]

Western Blot: Endo G Antibody (5F1) [NBP2-01696] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endo G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endo G.
Immunocytochemistry/Immunofluorescence: Endo G Antibody (5F1) [NBP2-01696] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Endo G.
Immunohistochemistry-Paraffin: Endo G Antibody (OTI5F1) [NBP2-01696]

Immunohistochemistry-Paraffin: Endo G Antibody (OTI5F1) [NBP2-01696]

Immunohistochemistry-Paraffin: Endo G Antibody (5F1) [NBP2-01696] - Staining of paraffin-embedded Human tonsil using anti-Endo G mouse monoclonal antibody.
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Applications for Endo G Antibody (OTI5F1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Endo G

The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq].

Alternate Names

EC 3.1.30.-, Endo G, endonuclease G, endonuclease G, mitochondrial, FLJ27463, mitochondrial endonuclease G

Entrez Gene IDs

2021 (Human)

Gene Symbol

ENDOG

Additional Endo G Products

Product Documents for Endo G Antibody (OTI5F1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Endo G Antibody (OTI5F1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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