Antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG. The immunogen is located within amino acids 40 - 90 of EndoG. Amino Acid Squence: GGPRGPGELAKYGLP

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 25843897)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endo G Antibody - BSA Free

Western Blot: Endo G AntibodyBSA Free [NBP1-76657]

Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human Endo G recombinant protein per lane. Antibodies: Endo G NBP1-76657, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1 ug/mL Lane 3: 2 ug/mL

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657]

Immunohistochemistry: Endo G Antibody [NBP1-76657] - Analysis of human pancreas tissue with EndoG antibody at 2.5 ug/ml.

Western Blot: Endo G AntibodyBSA Free [NBP1-76657]

Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation in Mouse 3T3 (M) and Human HepG2 (H) cell lysates. Loading: 15 ug of lysates per lane. Antibodies: Endo G NBP1-76657 (2 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: Endo G AntibodyBSA Free [NBP1-76657]

Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation in Human A431 Cell Lysate with the presence (A) or absence (B and C) of blocking peptide. Loading: 15 ug of lysates per lane. Antibodies: Endo G NBP1-76657 (A: 0.5 ug/mL, B: 0.5 ug/mL, C: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry-Paraffin: Endo G Antibody - BSA Free [NBP1-76657]

Immunohistochemistry-Paraffin: Endo G Antibody [NBP1-76657] - Human pancreas with anti-Endo G at 15 ug/ml.

Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657]

Immunofluorescence: Endo G Antibody [NBP1-76657] - Human pancreas tissue with EndoG antibody at 20 ug/mL. Green: EndoG Antibody Blue: DAPI staining

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] -

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] - Figure 6 Immunohistochemistry Validation of Endo G in Human Pancreas Tissue Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Endo G antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] -

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] - Figure 6 Immunohistochemistry Validation of Endo G in Human Pancreas Tissue
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Endo G antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] -

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] - Figure 5 Immunohistochemistry Validation of Endo G in Human Pancreas Tissue
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Endo G antibody at 15 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657] -

Immunocytochemistry/ Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657] - Figure 4 Immunofluorescence Validation of Endo G in Human Pancreas Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling Endo G with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657] -

Immunocytochemistry/ Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657] - Figure 4 Immunofluorescence Validation of Endo G in Human Pancreas TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling Endo G with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] -

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] - Figure 5 Immunohistochemistry Validation of Endo G in Human Pancreas Tissue Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Endo G antibody at 15 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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Applications for Endo G Antibody - BSA Free

Application

Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5-15 ug/mL

Western Blot

0.5-2 ug/mL

Application Notes

Use in ICC/IF reported in scientific literature (PMID:33473107)

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endo G

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.

Alternate Names

EC 3.1.30.-, Endo G, endonuclease G, endonuclease G, mitochondrial, FLJ27463, mitochondrial endonuclease G

Entrez Gene IDs

2021 (Human)

Gene Symbol

ENDOG

UniProt

Q14249 (Human)

Additional Endo G Products

Product Documents for Endo G Antibody - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Endo G Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Endo G Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76657

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all Endo G Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for Endo G Antibody - BSA Free

Western Blot: Endo G AntibodyBSA Free [NBP1-76657]

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657]

Western Blot: Endo G AntibodyBSA Free [NBP1-76657]

Western Blot: Endo G AntibodyBSA Free [NBP1-76657]

Immunohistochemistry-Paraffin: Endo G Antibody - BSA Free [NBP1-76657]

Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657]

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] -

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] -

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] -

Immunocytochemistry/ Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657] -

Immunocytochemistry/ Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657] -

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] -

Applications for Endo G Antibody - BSA Free

ELISA

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Endo G

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Endo G Products

Product Documents for Endo G Antibody - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Endo G Antibody - BSA Free