Endoglycan/PODXL2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37899
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (90%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: EAQSRIPWDSTQVICKDWSNLAGKNYIILNMTENIDCEVFRQHRGPQLLALVEEVLPRHGSGHHGAWHISLSKPSEKEQHLLMTLVGEQGVVPTQDVLSMLGDIRRSLEEIGIQNYSTTSS
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Endoglycan/PODXL2 Antibody
![Immunocytochemistry/ Immunofluorescence: Endoglycan/PODXL2 Antibody [NBP2-37899]](https://resources.bio-techne.com/images/products/Endoglycan-PODXL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-37899-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Endoglycan/PODXL2 Antibody [NBP2-37899]")
Immunocytochemistry/ Immunofluorescence: Endoglycan/PODXL2 Antibody [NBP2-37899]
Immunocytochemistry/Immunofluorescence: Endoglycan/PODXL2 Antibody [NBP2-37899] - Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.![Immunohistochemistry: Endoglycan/PODXL2 Antibody [NBP2-37899]](https://resources.bio-techne.com/images/products/Endoglycan-PODXL2-Antibody-Immunohistochemistry-NBP2-37899-img0002.jpg "Immunohistochemistry: Endoglycan/PODXL2 Antibody [NBP2-37899]")
Immunohistochemistry: Endoglycan/PODXL2 Antibody [NBP2-37899]
Immunohistochemistry: Endoglycan/PODXL2 Antibody [NBP2-37899] - Staining of human pancreas shows strong cytoplasmic and nuclear positivity in exocrine glandular cells.
Immunohistochemistry: Endoglycan/PODXL2 Antibody [NBP2-37899] -
Immunohistochemistry: Endoglycan/PODXL2 Antibody [NBP2-37899] -Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and leydig cells.Applications for Endoglycan/PODXL2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Endoglycan/PODXL2
Endoglycan, also named Podocalyxin-like 2 protein (PODLX2), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural and sequence similarity. It is expressed on hematopoietic precursors, endothelial cells, smooth muscle cells and leukocyte subpopulations. Endoglycan is a potential marker for hematopoietic progenitors. It also functions as an L-Selectin ligand and may be involved in the inhibition of stem cell differentiation and leukocyte-endothelial cell adhesion.
Additional Endoglycan/PODXL2 Products
Product Documents for Endoglycan/PODXL2 Antibody
Product Specific Notices for Endoglycan/PODXL2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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