Endoglycan/PODXL2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30066
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Between 300 and 350
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Endoglycan/PODXL2 Antibody
Immunoprecipitation:Endoglycan/PODXL2 AntibodyNBP3-30066] -
Immunoprecipitation:Endoglycan/PODXL2 AntibodyNBP3-30066] -Detection of human Endoglycan by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Endoglycan antibody NBP3-30066 (lot NBP3-30066-1) used for IP at 6 µg per reaction. Endoglycan was also immunoprecipitated by rabbit anti-Endoglycan antibody. For blotting immunoprecipitated Endoglycan, NBP3-30066 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for Endoglycan/PODXL2 Antibody
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate buffer, pH 7 to 8
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Endoglycan/PODXL2
Endoglycan, also named Podocalyxin-like 2 protein (PODLX2), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural and sequence similarity. It is expressed on hematopoietic precursors, endothelial cells, smooth muscle cells and leukocyte subpopulations. Endoglycan is a potential marker for hematopoietic progenitors. It also functions as an L-Selectin ligand and may be involved in the inhibition of stem cell differentiation and leukocyte-endothelial cell adhesion.
Alternate Names
PODLX2, PODXL2
Gene Symbol
PODXL2
Additional Endoglycan/PODXL2 Products
Product Specific Notices for Endoglycan/PODXL2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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