Endomucin/Mucin -14 Antibody (V.7C7.1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11257

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Product Datasheet / COA / SDS

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Flow Cytometry, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # V.7C7.1

Concentration

0.1 mg/ml

View all Endomucin Antibodies »

Product Summary for Endomucin/Mucin -14 Antibody (V.7C7.1)

Immunogen

Mouse endomucin-IgG fusion protein

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Applications for Endomucin/Mucin -14 Antibody (V.7C7.1)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

For Flow Cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Endomucin

The monoclonal antibody V.7C7.1 recognizes endomucin, type I membrane protein of 248 amino acids (75 kDa) and shows no signicant homology to any known glycoprotein. As a typical mucin-like glycoprotein, endomucin has a high content of serine and threonine residues, suggesting strong O-glycosylation; the sensitivity to O-sialoglycoprotein endopeptidase indicates that endomucin is also a sialomucin. Endomucin is an endothelial-specific sialomucin. It is a constitutively expressed endothelial cell surface protein that is found on all venules but is absent from high endothelial venule cells (HEV) of peripheral and mesenteric lymph nodes as well as Peyer's patches, the specialized site for most efficient lymphocyte trafficking. This could indicate an anti-adhesive function of endomucin, as demonstrated for other sialomucins. Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is another cell adhesion molecule that contains a mucin-like domain and is expressed on HEV in Peyer's patches, mesenteric lymph nodes and on venules in intestinal lamina propria. In the HEV of mesenteric lymph nodes, the mucin-like domain of a subpopulation of MAdCAM-1 molecules contains sulfated carbohydrate side chains that interact with L-selectin. The presence of three putative protein kinase C phosphorylation sites in the cytoplasmic tail of endomucin indicates that endomucin has the capacity to be a signaling molecule.

Alternate Names

EMCN, EMCN2, MUC-14

Gene Symbol

EMCN

Additional Endomucin Products

Product Documents for Endomucin/Mucin -14 Antibody (V.7C7.1)

Product Datasheets

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COA

SDS

Product Specific Notices for Endomucin/Mucin -14 Antibody (V.7C7.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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