Endomucin/Mucin -14 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03542
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Endomucin/Mucin -14 Antibody - BSA Free
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-135 of human Endomucin/Mucin -14 (NP_057326.2). NSTGVLEAANNSLVVTTTKPSITTPNTESLQKNVVTPTTGTTPKGTITNELLKMSLMSTATFLTSKDEGLKATTTDVRKNDSIISNVTVTSVTLPNAVSTLQSSKPKTETQSSIKTT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Endomucin/Mucin -14 Antibody - BSA Free
![Western Blot: Endomucin/Mucin -14 AntibodyBSA Free [NBP3-03542]](https://resources.bio-techne.com/images/products/Endomucin-Mucin--14-Antibody-Western-Blot-NBP3-03542-img0001.jpg "Western Blot: Endomucin/Mucin -14 AntibodyBSA Free [NBP3-03542]")
Western Blot: Endomucin/Mucin -14 AntibodyBSA Free [NBP3-03542]
Western Blot: Endomucin/Mucin -14 Antibody [NBP3-03542] - Analysis of extracts of various cell lines, using Endomucin/Mucin -14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Applications for Endomucin/Mucin -14 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Endomucin
Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80 - 120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule.
Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21 - 190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD of Endomucin. One shows a deletion of aa 91 - 141, a second shows a one aa substitution for aa 91 - 129, and a third shows a one aa substitution for aa 129 - 142. Over aa 21 - 90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.
Alternate Names
EMCN, EMCN2, MUC-14
Gene Symbol
EMCN
Additional Endomucin Products
Product Documents for Endomucin/Mucin -14 Antibody - BSA Free
Product Specific Notices for Endomucin/Mucin -14 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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