Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A7L6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47695
![Immunohistochemistry-Paraffin: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A7L6) - Azide and BSA Free [NBP2-47695]](https://resources.bio-techne.com/images/products/Endorepellin-Perlecan-Heparan-Sulfate-Proteoglycan-Antibody-A7L6-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47695-img0001.jpg "Immunohistochemistry-Paraffin: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A7L6) - Azide and BSA Free [NBP2-47695]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Porcine, Bovine, Fish, Monkey
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # A7L6
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary
Immunogen
Murine EHS laminin preparation
Reactivity Notes
Use in Human reported in scientific literature (PMID:34228647)
Localization
Basement membrane
Specificity
This monoclonal antibody specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecans strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Theoretical MW
400 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-44448).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images
Immunohistochemistry-Paraffin: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A7L6) - Azide and BSA Free [NBP2-47695]
Immunohistochemistry-Paraffin: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A7L6) - Azide and BSA Free [NBP2-47695] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Heparan Sulfate Monoclonal Antibody (A7L6).Applications
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Endorepellin/Perlecan
Long Name
Heparan Sulfate Proteoglycan
Alternate Names
HSPG2, Perlecan
Gene Symbol
HSPG2
Additional Endorepellin/Perlecan Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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