Endothelin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87942

Novus Biologicals, part of Bio-Techne
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NBP1-87942
NBP1-87942-25ul
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Endothelin-2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LPRRCQCSSARDPACATFCLRRPWTEAGAVPSRKSPADVFQTGKTGATTGELLQRLRDISTVKSLFAKRQQEAMREPRSTHSRWR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Endothelin 2 Antibody

Western Blot: Endothelin 2 Antibody [NBP1-87942]

Western Blot: Endothelin 2 Antibody [NBP1-87942]

Western Blot: Endothelin 2 Antibody [NBP1-87942] - Analysis in control (vector only transfected HEK293T lysate) and EDN2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Endothelin 2 Antibody [NBP1-87942]

Immunocytochemistry/ Immunofluorescence: Endothelin 2 Antibody [NBP1-87942]

Immunocytochemistry/Immunofluorescence: Endothelin 2 Antibody [NBP1-87942] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: Endothelin 2 Antibody [NBP1-87942]

Immunohistochemistry-Paraffin: Endothelin 2 Antibody [NBP1-87942]

Immunohistochemistry-Paraffin: Endothelin 2 Antibody [NBP1-87942] - Staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.

Applications for Endothelin 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 24913676). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endothelin-2

Consisting of vasoconstrictive peptides, Endothelin 2, or END2 is a member of the endothelin/sarafotoxin family. Widely expressed in all tissues, Endothelin 2 initiates many intracellular signaling events and is involved in the maintenance of vascular tone. This gene is also involved in a variety of biological processes, with the two most common being hypertension and ovulation.

Alternate Names

EDN2, Endothelin2, ET2, PPET2

Gene Symbol

EDN2

Additional Endothelin-2 Products

Product Documents for Endothelin 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Endothelin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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