Endothelin-3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03508

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Endothelin-3 Antibodies »

Product Summary for Endothelin-3 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 17-187 of human Endothelin-3 (NP_996917.1). AGFVPCSQSGDAGRRGVSQAPTAARSEGDCEETVAGPGEETVAGPGEGTVAPTALQGPSPGSPGQEQAAEGAPEHHRSRRCTCFTYKDKECVYYCHLDIIWINTPEQTVPYGLSNYRGSFRGKRSAGPLPGNLQLSHRPHLRCACVGRYDKACLHFCTQTLDVSSNSRTAE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Endothelin-3 Antibody - BSA Free

Western Blot: Endothelin-3 AntibodyBSA Free [NBP3-03508]

Western Blot: Endothelin-3 AntibodyBSA Free [NBP3-03508]

Western Blot: Endothelin-3 Antibody [NBP3-03508] - Analysis of extracts of A-549 cells, using Endothelin-3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.

Applications for Endothelin-3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Endothelin-3

Endothelin 3 is encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Four alternatively spliced transcript variants encoding three distinct isoforms have been observed. [provided by RefSeq]

Alternate Names

EDN3, Endothelin3

Gene Symbol

EDN3

Additional Endothelin-3 Products

Product Documents for Endothelin-3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Endothelin-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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