ENKD1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85147
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Rat (92%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QVLAQVLEQQRQAQEHYNATQKGHVPHYLLERRDLWRREAEARKQSQPDPAMPPGHTRMPENQRLETLTKL
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%). Reactivity reported in scientific literature (PMID: 23435261)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ENKD1 Antibody
Western Blot: ENKD1 Antibody [NBP1-85147]
Western Blot: ENKD1 Antibody [NBP1-85147] - Analysis in control (vector only transfected HEK293T lysate) and ENKD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: ENKD1 Antibody [NBP1-85147]
Immunocytochemistry/Immunofluorescence: ENKD1 Antibody [NBP1-85147] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.Immunohistochemistry-Paraffin: ENKD1 Antibody [NBP1-85147]
Immunohistochemistry-Paraffin: ENKD1 Antibody [NBP1-85147] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.Applications for ENKD1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ENKD1
Alternate Names
C16orf48, chromosome 16 open reading frame 48, DAKV6410, DKFZP434A1319, hypothetical protein LOC84080
Gene Symbol
ENKD1
Additional ENKD1 Products
Product Documents for ENKD1 Antibody
Product Specific Notices for ENKD1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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