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ENKD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85147

Novus Biologicals, part of Bio-Techne
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NBP1-85147
NBP1-85147-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QVLAQVLEQQRQAQEHYNATQKGHVPHYLLERRDLWRREAEARKQSQPDPAMPPGHTRMPENQRLETLTKL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%). Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ENKD1 Antibody

Western Blot: ENKD1 Antibody [NBP1-85147]

Western Blot: ENKD1 Antibody [NBP1-85147]

Western Blot: ENKD1 Antibody [NBP1-85147] - Analysis in control (vector only transfected HEK293T lysate) and ENKD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ENKD1 Antibody [NBP1-85147]

Immunocytochemistry/ Immunofluorescence: ENKD1 Antibody [NBP1-85147]

Immunocytochemistry/Immunofluorescence: ENKD1 Antibody [NBP1-85147] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: ENKD1 Antibody [NBP1-85147]

Immunohistochemistry-Paraffin: ENKD1 Antibody [NBP1-85147]

Immunohistochemistry-Paraffin: ENKD1 Antibody [NBP1-85147] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.

Applications for ENKD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ENKD1

Alternate Names

C16orf48, chromosome 16 open reading frame 48, DAKV6410, DKFZP434A1319, hypothetical protein LOC84080

Gene Symbol

ENKD1

Additional ENKD1 Products

Product Documents for ENKD1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ENKD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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