ENO3 Antibody (003)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90274
Recombinant Monoclonal Antibody
![Western Blot: ENO3 Antibody (003) [NBP2-90274]](https://resources.bio-techne.com/images/products/ENO3-Antibody-003-Western-Blot-NBP2-90274-img0002.jpg "Western Blot: ENO3 Antibody (003) [NBP2-90274]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 003
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human ENO3 (Accession#: AAH17249.1; Met1-Lys434).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ENO3 Antibody (003)
Western Blot: ENO3 Antibody (003) [NBP2-90274]
Western Blot: ENO3 Antibody (003) [NBP2-90274] - Lane A: HepG2 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: Hela Whole Cell LysateLane D: MOLT-4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:47 kDaObserved band size:50 kDa![Immunohistochemistry-Paraffin: ENO3 Antibody (003) [NBP2-90274]](https://resources.bio-techne.com/images/products/ENO3-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90274-img0001.jpg "Immunohistochemistry-Paraffin: ENO3 Antibody (003) [NBP2-90274]")
Immunohistochemistry-Paraffin: ENO3 Antibody (003) [NBP2-90274]
Immunohistochemistry-Paraffin: ENO3 Antibody (003) [NBP2-90274] - Staining of human ENO3 in human skeletal muscle with rabbit monoclonal antibody (1:200).![Immunoprecipitation: ENO3 Antibody (003) [NBP2-90274]](https://resources.bio-techne.com/images/products/ENO3-Antibody-003-Immunoprecipitation-NBP2-90274-img0003.jpg "Immunoprecipitation: ENO3 Antibody (003) [NBP2-90274]")
Immunoprecipitation: ENO3 Antibody (003) [NBP2-90274]
Immunoprecipitation: ENO3 Antibody (003) [NBP2-90274] - Lane A:0.5 mg Raji Whole Cell Lysate2 uL anti-ENO3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ENO3 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 47 kDaObserved band size: 53 kDaApplications for ENO3 Antibody (003)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ENO3
Alternate Names
2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, beta-enolase, EC 4.2.1, EC 4.2.1.11, Enolase 3, enolase 3 (beta, muscle), enolase 3, (beta, muscle), GSD13, MSE, Muscle-specific enolase, Skeletal muscle enolase
Gene Symbol
ENO3
Additional ENO3 Products
Product Documents for ENO3 Antibody (003)
Product Specific Notices for ENO3 Antibody (003)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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