Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # ENO2/1462

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Enolase 2/Neuron-specific Enolase Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Enolase 2/Neuron-specific Enolase (around aa416-433) (exact sequence is proprietary) (Uniprot: P09104)

Localization

Cytoplasmic

Specificity

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Marker

Neuroendocrine Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-59602).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (ENO2/1462).
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603] - Formalin-fixed, paraffin-embedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1462).
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with NSE gamma Monoclonal Antibody (ENO2/1462)
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Applications

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Enolase 2/Neuron-specific Enolase

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Gene Symbol

ENO2

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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