Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59603
![Protein Array: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]](https://resources.bio-techne.com/images/products/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Azide-and-BSA-Free-Protein-Array-NBP2-59603-img0001.jpg "Protein Array: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]](https://resources.bio-techne.com/images/products/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-59603-img0004.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
CyTOF-ready, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # ENO2/1462
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary
Immunogen
A synthetic peptide of human Enolase 2/Neuron-specific Enolase (around aa416-433) (exact sequence is proprietary) (Uniprot: P09104)
Localization
Cytoplasmic
Specificity
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Marker
Neuroendocrine Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-59602).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (ENO2/1462).![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]](https://resources.bio-techne.com/images/products/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-59603-img0002.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]")
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603] - Formalin-fixed, paraffin-embedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1462).![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]](https://resources.bio-techne.com/images/products/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-59603-img0003.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]")
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603]
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with NSE gamma Monoclonal Antibody (ENO2/1462)Applications
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Protein Array
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Enolase 2/Neuron-specific Enolase
Alternate Names
ENO2, gamma-Enolase, Neuronspecific Enolase, NSE
Gene Symbol
ENO2
Additional Enolase 2/Neuron-specific Enolase Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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