ENOX2 Antibody (5P7H2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33316

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 5P7H2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ENOX2 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 411-610 of human ENOX2 (NP_872114.1).

Sequence:
AEALKEENDSLRWQLDAYRNEVELLKQEQGKVHREDDPNKEQQLKLLQQALQGMQQHLLKVQEEYKKKEAELEKLKDDKLQVEKMLENLKEKESCASRLCASNQDSEYPLEKTMNSSPIKSEREALLVGIISTFLHVHPFGASIEYICSYLHRLDNKICTSDVECLMGRLQHTFKQEMTGVGASLEKRWKFCGFEGLKLT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ENOX2 Antibody (5P7H2)

ENOX2 Antibody (5P7H2)

Western Blot: ENOX2 Antibody (5P7H2) [NBP3-33316] -

Western Blot: ENOX2 Antibody (5P7H2) [NBP3-33316] - Western blot analysis of lysates from MCF7 cells, using ENOX2 Rabbit mAb at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:200000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.

Applications for ENOX2 Antibody (5P7H2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ENOX2

The protein encoded by the ENOX2 gene is a growth-related cell surface protein. It was identifed because it reacts with the monoclonal antibody KI in cells, such as the ovarian carcinoma line OVCAR-3, also expressing the CAKI surface glycoprotein. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The two activities alternate with a period length of about 24 minutes. The encoded protein also displays prion-like properties. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Alternate Names

COVA1, Cytosolic ovarian carcinoma antigen 1APK1 antigen, ecto-NOX disulfide-thiol exchanger 2, tNOXAPK1, Tumor-associated hydroquinone oxidase

Gene Symbol

ENOX2

Additional ENOX2 Products

Product Documents for ENOX2 Antibody (5P7H2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ENOX2 Antibody (5P7H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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