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ENT1 Antibody (PD00-77)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32309

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32309

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PD00-77

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within C-terminus of human ENT1. (Uniprot: Q99808)

Localization

Basolateral cell membrane, Apical cell membrane, Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ENT1 Antibody (PD00-77)

ENT1 Antibody (PD00-77)

Immunohistochemistry: ENT1 Antibody (PD00-77) [NBP3-32309]

Immunohistochemistry: ENT1 Antibody (PD00-77) [NBP3-32309] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-ENT1 antibody (NBP3-32309) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32309) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for ENT1 Antibody (PD00-77)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ENT1

ENT1 is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]

Long Name

Equilibrative nucleoside transporter 1

Alternate Names

SLC29A1

Gene Symbol

SLC29A1

Additional ENT1 Products

Product Documents for ENT1 Antibody (PD00-77)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ENT1 Antibody (PD00-77)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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