EOGT/AER61 Antibody (JE64-87)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32007
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE64-87
Concentration
1 mg/ml
Product Summary for EOGT/AER61 Antibody (JE64-87)
Immunogen
Recombinant protein within Human EOGT/AER61 aa 1-200 / 527. (Uniprot: Q5NDL2)
Localization
Endoplasmic reticulum lumen.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for EOGT/AER61 Antibody (JE64-87)
Western Blot: EOGT/AER61 Antibody (JE64-87) [NBP3-32007]
Western Blot: EOGT/AER61 Antibody (JE64-87) [NBP3-32007] - Western blot analysis of EOGT/AER61 on different lysates with Rabbit anti-EOGT/AER61 antibody (NBP3-32007) at 1/2,000 dilution.Lane 1: Neuro-2a cell lysate (20 ug/Lane)
Lane 2: U-87 MG cell lysate (20 ug/Lane)
Lane 3: HeLa cell lysate (20 ug/Lane)
Lane 4: Caco-2 cell lysate (20 ug/Lane)
Lane 5: HDLM-2 cell lysate (20 ug/Lane)
Lane 6: HepG2 cell lysate (20 ug/Lane)
Lane 7: Mouse brain tissue lysate (40 ug/Lane)
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32007) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for EOGT/AER61 Antibody (JE64-87)
Application
Recommended Usage
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EOGT
Long Name
EGF Domain-specific O-linked N-acetylglucosamine (GlcNAc) Transferase
Alternate Names
AER61, C3orf64
Gene Symbol
EOGT
Additional EOGT Products
Product Documents for EOGT/AER61 Antibody (JE64-87)
Product Specific Notices for EOGT/AER61 Antibody (JE64-87)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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